Workshop Highlights | Hands On with xCELLigence RTCA and Seahorse XF Analyzer

Last week, we wrapped up our joint-lab workshop in collaboration with Agilent Technologies – “Optimizing Cell Therapy Workflows: Hands-On with xCELLigence RTCA and Seahorse Technology."

The full-day session brought participants through both lecture and hands-on sessions, equipping them with practical knowledge on Agilent’s powerful platforms for real-time cell analysis.

The morning featured lectures on Agilent's xCELLigence RTCA and Seahorse XF Analyzer, followed by practical sessions in the afternoon. Participants worked through workflows including background reading, cell seeding, initiating experiments, and software analysis for cell proliferation and potency using the xCELLigence RTCA system.

They also explored Seahorse XF Analyzer procedures— learning to evaluate mitochondrial respiration and glycolysis through real-time OCR, PER, and ECAR measurements. Participants were guided through essential steps such as cartridge hydration, cell seeding, drug ports loading, and software analysis.

The workshop concluded with a short quiz to reinforce participants’ knowledge and understanding of the day’s content.

A big thank you to the trainers Chin-Chieh (JJ) Loh and Dr Federica Tomay, PhD, MSc from Agilent Technologies for guiding the sessions and sharing your invaluable expertise. And to all our participants – thank you for your enthusiasm! We hope you enjoyed the experience and left with fresh expertise to apply in your cell therapy product characterisation workflow.